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Generation of HFF cells with stable SPOC1 overexpression. (A) Western blot analysis for detection of SPOC1 in lysates of control (HFF/control) and SPOC1-overexpressing (HFF/SPOC1) HFFs using an antibody against endogenous SPOC1 (anti-SPOC1 rat). (B) Immunofluorescence staining of SPOC1 in HFF/control and HFF/SPOC1 cells, using the SPOC1 mAb. Cell nuclei were counterstained with DAPI (4′,6-diamidino-2-phenylindole).
