FIG 8.

Hemagglutination capacities of rsT3SA⁺ and σ1 coiled-coil-to-body transition mutant reoviruses. Equal numbers of particles of purified reovirus virions (V) or ISVPs (I) were serially diluted, incubated with a 1% suspension of human erythrocytes at 4°C for 4 h, and scored for HA. Erythrocyte shields indicated HA, and erythrocyte buttons indicated the absence of erythrocyte cross-linking. Results are expressed as mean log₂ HA titers for two replicates from each of two independent experiments. The HA titer is defined as 2.5 × 10¹⁰ particles divided by the number of particles per HA unit. One HA unit equals the minimum number of particles sufficient to produce HA. ND denotes samples for which HA was not detected at the highest number of particles (2.5 × 10¹⁰). Error bars represent standard deviations. Values that differ significantly from those for rsT3SA⁺ under each condition by one-way ANOVA with Dunnett's multiple-comparison test are indicated with * (P < 0.001).