FIG 2.

Induction of TRIM21 depends on the JAK/STAT signaling pathway. (A) HLCZ01 cells were stably transfected with either scrambled shRNA (sh-con) or IFNAR shRNA (sh-IFNAR). (Left) IFNAR1 mRNA was analyzed by real-time PCR and normalized with GAPDH. (Right) IFNAR1 protein was analyzed by immunoblotting. (B) Immunoblot analysis of the indicated proteins in HLCZ01-sh-con and HLCZ01-sh-IFNAR1 cell lines treated with IFN-α (500 U/ml) for 30 min. (C) HLCZ01 cells stably transfected with scrambled shRNA or IFNAR shRNA were treated with IFN-α (100 U/ml) for 6 h. TRIM21 mRNA was determined by real-time PCR and normalized with GAPDH. (D and E) TRIM21 protein was analyzed by immunoblotting. HLCZ01 cells stably transfected with scrambled shRNA or IFNAR shRNA were transfected with HCV 3′ UTR (D) or poly(I·C) (E) for 6 h. TRIM21 mRNA and protein were analyzed by real-time PCR and immunoblotting, respectively. (F and G) HLCZ01 cells stably transfected with scrambled shRNA or IFNAR shRNA were infected with NDV (F) or SeV (G) for 16 h. TRIM21 mRNA was determined by real-time PCR and normalized with GAPDH. TRIM21 protein was detected by immunoblotting. The results are presented as means ± standard deviations. *, P < 0.05; **, P < 0.01 versus control.