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. 2018 Jun 29;92(14):e00321-18. doi: 10.1128/JVI.00321-18

FIG 3.

FIG 3

TRIM21 positively regulates innate immune response to RNA nucleic acid mimics in HLCZ01 cells. (A to D) HLCZ01 cells were transfected with p3X-Flag-CMV-vector or p3X-Flag-TRIM21 plasmid (A and C) or pSilencer-vector or pSilencer-TRIM21 plasmid (B and D) for 48 h and then transfected with 200 ng of HCV 3′ UTR (A and B) or 200 ng of poly(I·C) (C and D) for the indicated times. The levels of IFN-β, IL-28A, IL-29, TNF-α, IL-6, and ISG12a mRNAs were analyzed by real-time PCR and normalized with GAPDH. The results are presented as means ± standard deviations. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus control. F, Flag.