FIG 5.

TRIM21 positively regulates innate immune response to RNA viral infection in HLCZ01 cells. (A to D) HLCZ01 cells were transfected by p3X-Flag-CMV-vector or p3X-Flag-TRIM21 (A and C) or pSilencer-vector or pSilencer-TRIM21 (B and D) for 24 h and then infected with NDV (A and B) or SeV (C and D) for the indicated times. The levels of IFN-β, IL-28A, IL-29, TNF-α, IL-6, and ISG12a mRNAs were tested by real-time PCR and normalized with GAPDH. The results are presented as means ± standard deviations. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus control.