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. 2018 Jun 29;92(14):e00321-18. doi: 10.1128/JVI.00321-18

FIG 6.

FIG 6

TRIM21 positively regulates innate immune response to RNA viral infection in Huh7 cells. (A to D) Luciferase activity of lysates in Huh7 cells cotransfected with IFN-β–Luc or ISRE-Luc and p3X-Flag-CMV-vector or p3X-Flag-TRIM21 (A and C) or pSilencer-vector or pSilencer-TRIM21 (B and D) for 12 h and then infected with NDV (A and B) or SeV (C and D) for 12 h. The results are presented relative to the luciferase activity in control cells. (E and F) Luciferase activity of lysates in Huh7.5.1(MAVS-C508R) cells infected with HCV (MOI = 2) for 48 h, followed by cotransfection with IFN-β–Luc or ISRE-Luc and p3X-Flag-CMV vector or p3X-Flag-TRIM21 (E) or pSilencer-vector or pSilencer-TRIM21 (F) for 24 h. (G) Huh7 cells were transfected with p3X-Flag-CMV vector or p3X-Flag-TRIM21 or with pSilencer-vector or pSilencer-TRIM21 for 24 h and then infected with HSV for 24 h. The level of IFN-β mRNA was tested by real-time PCR and normalized with GAPDH. The results are presented as means ± standard deviations. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus control.