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. 2018 Jun 29;92(14):e00538-18. doi: 10.1128/JVI.00538-18

FIG 2.

FIG 2

Glycan binding specificities of P[6]/P[19]/P[4]/P[8] VP8*s. VP8* proteins of human P[19], porcine P[19], porcine P[6] (z84), a porcine P[6] mutant, porcine-like human P[6] (LL3354), the prevalent human P[6] (5311142), a human P[6] (5311142) mutant, human P[4] (11151099), and human P[8] (11221075) were subjected to the microtiter plate binding assay. GST protein was used as the negative control. (a) SDS-PAGE of the GST-VP8* fusion proteins. The red arrows indicate the proteins of interest. The 26-kDa protein was free GST. (b) ELISA-based assay of P[6] VP8* protein binding to two concentrations of mucin core 2 (0.2 μg and 2 μg). (c) ELISA-based assay of P[19]/P[4]/P[8] VP8* protein binding to mucin core 2 (0.2 μg and 2 μg). (d) ELISA-based assay of VP8* protein binding to LNT (Galβ1-3GlcNAcβ1-3Galβ1-4Glc) at 0.2 μg. The error bars indicate standard errors from triplicate repeats.