FIG 1.

STAT3 localizes to promoters and regulates expression of KRAB-ZFP genes. (A and B) Levels of SZF1 (A) and ZNF557 (B) mRNAs in healthy-donor-derived LCLs and AD-HIES patient-derived LCLs were determined by qRT-PCR. (C to E) Levels of SZF1, ZNF557, and STAT3 mRNAs in EBV⁺ latent BL cells (C), healthy-donor-derived LCLs (D), and AD-HIES patient-derived LCLs (E) transfected with scrambled siRNA (open bars) or siSTAT3 (black bars) were quantitated by qRT-PCR. Data represent means of three independent experiments (C) or three separate LCLs (A, B, D, and E). (F and G) Relative amounts of SZF1 (F) and ZNF557 (G) promoter DNAs precipitated by anti-STAT3 antibody were determined by qPCR with normalization to input DNA. BL cells were left untreated or treated with NaB for 24 h and examined by qPCR using primers spanning bioinformatically predicted STAT3-binding sites in the SZF1 promoter (site 1, position −1463; site 2, position −1226; site 3, position −74 [relative to transcription start site]) or a non-STAT3-binding site on the SZF1 promoter (position −500 relative to the transcription start site) or the ZNF557 promoter (−255 relative to transcription start site). Data represent the averages of results from three independent experiments; error bars indicate standard errors of the means (*, p ≤ 0.05; NS, not significant; p, promoter).