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. 2018 Jun 29;92(14):e00633-18. doi: 10.1128/JVI.00633-18

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FIG 1 — DENV infection of HUVEC and MDM induces FH mRNA but not protein. HUVEC and MDM were isolated and were left uninfected or DENV infected at MOI of 1 and 3, respectively. (A, B) At 24 and 48 hpi, supernatants and total cellular RNA were collected from HUVEC (A) and MDM (B) and analyzed by plaque assay (PA; bars) and RT-PCR (lines), with the values being normalized against those for cyclophilin. (C, D) Cell lysates of HUVEC (C) of MDM (D) were analyzed for FH mRNA by RT-PCR, with the values being normalized against those for cyclophilin. (E, F) Supernatants of HUVEC (E) of MDM (F) were analyzed by ELISA for FH protein. Results represent the mean ± standard deviation for duplicate samples and are representative of those from three independent infection experiments. *, P < 0.05, Student's unpaired t test. UI, uninfected; DV, dengue virus.