FIG 3.

The C-terminal BR is important for AAP1 function but can be replaced by heterologous NoLS. (A) Schematic of different AAP mutants. The T/S of AAP1 was replacing by EGFP (AAP1E). The BR was replaced by RNase P subunit 29 NoLS (Rpp29), adaptor protein in AP-3 complex NoLS (AP3D1), virus-derived SV40 NLS (SV40), and HIV Rev NoLS domain (HIV Rev). (B) Representative Western blot images of VP and AAP expression as described in Fig. 1B. (C) Relative vector yields using different AAP constructs normalized to wild-type AAP1 (AAP1). (D) Transduction of rAAV1 packaging luciferase produced from different AAP1 constructs as described above. RLUs were normalized to AAP1. Error bars were determined and statistical analyses were performed as described above.