FIG 5.

Domain deletion analysis of the N terminus of AAP1 reveals that the HR and CC are important for AAP functions. (A) Schematic of different AAP mutants. The N-terminal domains are either deleted or replaced with their AAP5 counterparts (purple). All AAP mutants have a C9 epitope tag at the C terminus. (B) Representative Western blot image of viral proteins, AAPs, and actin expression of HEK293 cells transfected by quadruple transfection for rAAV production after 3 days posttransfection. (C) Relative vector yields using different AAP constructs normalized to wild-type AAP1 (AAP1). (D) Transduction of rAAV1 packaging luciferase produced from different AAP1 constructs as described above. RLUs were normalized to AAP1. Error bars were determined and statistical analyses were performed as described above.