FIG 8.

Replacement of T/S with oligomerization domains. (A) Schematic of different AAP mutants. The T/S was replaced by different oligomerization domains: the collagen trimerization domain (Collagen), a synthetic tetramerization domain (Tetra), or the influenza A/WSN/33 HA coiled-coil domain (WSN) and AAP1 as a control. All AAP mutants have a C9 epitope tag at the C terminus. (B) Representative Western blot image of VP and AAP expression as described in Fig. 1B. The asterisk (*) lane is transfected with 2× AAP1 plasmid amount compared to lane 1, and the red arrow denotes the position of AAP1. (C) Relative vector yield using different AAP constructs normalized to wild-type AAP1 (AAP1). Error bars were determined and statistical analyses were performed as described above.