TABLE 2.

Summary of biological properties of T/S and N-terminal constructs^a

Constructb	AAP level	VP level	Vector yield	Interaction with VP
T/S region
AAP1 WT	WT	WT	WT	Yes
TL1	++	WT	+	NA
TL2	WT	−	−−−	NA
TL3	WT	−	−−−	NA
TL4	−	−	−−−	NA
Silk	++	WT	+	NA
SilkE1	+	WT	+	NA
Full MLD	WT	−	WT	NA
Partial MLD	+++	WT	+	NA
AAP1E	++++	+	WT	Yes
AAP1-Collagen (21)	++++++	++	+++	NA
AAP1-Tetra (22)	+++++	+	+	NA
AAP1-WSN	++	WT	−	NA
N terminus
AAP1 WT	WT	WT	WT	Yes
AAP1E	++++	+	WT	Yes
AAP5E	++++	− −	−−−	Yes
ΔHR	++++++	− −	−−−	No
5HR	++++	− −	−−−	Yes
ΔCC	++++	− −	−−−	No
5CC	+	−	− −	Yes
ΔPRR	++++	WT	− −	Yes
5PRR	WT	WT	WT	Yes

^aWT represents similar properties, “−” represents reduced levels, and “+” represents increased levels compared to AAP1 WT. The numbers of “+” and “−” are proportional to the increase or decrease in expression levels or titer. NA, not applicable.

^bThe numbers in parentheses indicate references for the sequences.