FIG 6.

Hemosiderin pigment deposition in CM brain. All panels show a similar region of cerebellum from CWD-inoculated CM270. Staining methods are shown in the lower left corner of each panel. (A to D) Brown pigments (yellow arrows) were observed in slides stained with 3F4 (A), no-primary-antibody controls (B), and H&E-stained sections (C and D) of brain tissues examined from all the CWD-inoculated and uninoculated CM. (E) Prussian blue iron staining (blue) on adjacent sections confirmed that the pigment was hemosiderin. Hemosiderin pigment deposition was common near Purkinje cells, often near blood vessels (red arrow). The bar shown in panel A is 25 μm and applies to all panels.