FIG 7.

Detection of PrP in spinal cords and nerve roots of CM using anti-PrP antibody (6H4). Spinal cord regions (cervical, thoracic, and lumbar) were screened for PrP deposition in 6 CWD-inoculated CM, 2 uninoculated CM, and 1 normal elk brain-inoculated CM. (A to E) Examples of thoracic spinal cord from one uninoculated CM (A), a normal elk brain-inoculated CM (C), and three CWD-inoculated CM (B, D, and E). PrP staining (brown) was similar for all CM. The strongest staining was located in the substantia gelatinosa region of the dorsal horn (blue arrow). (F and H) PrP staining (purple arrows) was also observed in nerve roots adjacent to the spinal cord. (G) The same nerve region depicted in panel F, without antibody 6H4 being applied (no primary ab). The bar shown in panel A is 1 mm and applies to panels A to E; the bar shown in panel F is 50 μm and applies to panels F to H.