FIG 2.

ASC sorting and frequency of VZV-specific ASCs. Five older adults were vaccinated with Zostavax, and PBMCs were collected on day 7. (A) ASCs were single-cell sorted into PCR plates using a FACSAria II (left and middle panels, presort; right panel, postsort to assess purity). (B) The frequency of VZV-specific ASCs was calculated via IgG ELISPOT assay. Top row, VZV-specific IgG ASCs; bottom row, total IgG ASCs. The percentages of specific ASCs were measured by taking the number of VZV-specific IgG ASCs divided by the total number of IgG ASCs detected. The number of ASCs plated per well is listed.