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Work flow to identify glycoprotein-specific VZV monoclonal antibodies. Supernatants generated using ASCs single cell sorted, cloned, and expressed in Expi293 cells were tested for VZV lysate binding via ELISA. VZV glycoprotein-positive but non-gE antibodies (10/25) were tested via immunoprecipitation followed by LC/MS to determine the specificity. All glycoprotein-positive antibodies were then tested for neutralization and cell-to-cell spread inhibition in vitro.
