FIG 5.

Identification of non-gE-specific monoclonal antibodies. Immunoprecipitation followed by nano-LC-MS/MS was done using the gP-specific monoclonal antibodies and the top three MS hits listed. (A) VZV gB-specific monoclonal antibodies. (B) VZV gH-specific monoclonal antibodies. (C) VZV gI-specific monoclonal antibodies. (D) Western blotting with RM-1D1, 302-1B12, 302-1C12, 303-1C1, and 303-1B2 antibodies was performed using a glycoprotein lysate (including all VZV glycoproteins). The table shows the predicted molecular weights and pIs. (E) Of the 25 VZV glycoprotein-positive antibodies, 15 were gE specific, 5 were gI specific, 3 were gB specific, and 2 were gH specific.