FIG 2.

Effect of HCV infection on ApoE. (A) Stable Huh7 cells that expressed GFP-LC3 were mock infected or infected with HCV, fixed at the time points indicated, and immunofluorescence stained for ApoE (red) and nuclei (blue). Arrows denote the limited colocalization of ApoE with GFP-LC3 puncta. (B and C) Huh7 cells were infected with HCV (MOI = 1) and harvested at the different time points for immunoblot analysis (B) or real-time RT-PCR analysis for ApoE mRNA (C). For panel C, the ApoE mRNA level was normalized against the GAPDH RNA, which served as the internal control. The ApoE mRNA level in mock-infected cells was arbitrarily defined as 1. The experiments were repeated at least three times for statistical analysis.