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Alteration of subcellular localization of ApoE by ATG7 knockdown. (A) Huh7 cells were electroporated with GND or JFH-1 RNA for 24 h and then transfected with control siRNA (siNC) or ATG7 siRNA (siATG7). Cells were fixed at 48 h after siRNA transfection and stained for HCV E2 (green), ApoE (red), or nuclei (blue). (B) Percentage of ApoE puncta that were positive for HCV E2 (i.e., yellow/red ratio). The results represent the average for >50 cells. ***, P < 0.001.
