FIG 7.

Effects of ApoE on the colocalization of HCV E2 and ApoE and progeny HCV titers. Huh7 cells were infected with HCV (MOI = 1) and were treated with DMSO or 100 nM BafA1 for 16 h prior to the analysis. (A) Cells were fixed at 24 h postinfection and stained for HCV E2 (green), ApoE (red), and nuclei (blue). (B) The incubation media were collected for analysis of ApoE using ELISA. (C to E) Cells and the incubation media were harvested at 24 h postinfection for quantification of intracellular HCV titers (C), extracellular HCV titers (D), and extracellular HCV RNA (E). **, P < 0.01; ***, P < 0.001.