FIG 3.

ACYPI003649, a second RR-1 protein identified in the acrostyle. (A) Alignments of Ap_ACYPI009006 (Ap_Stylin-01) and Ap_ACYPI003649 were performed using T-Coffee software (66, 67). Shading was done with BOXSHADE 3.21 software. Identical residues are shaded in black; similar residues are shaded in gray. The consensus sequence is shown at the bottom, with periods indicating conserved substitutions and asterisks indicating identities. The signal peptide (SP) cleavage site is indicated. The line above the sequence delineates peptide 1-11. (B) Immunolabeling of A. pisum stylets with anti-1-07 antibody. Scale bars represent 5 μm.