TABLE 1.

Antibodies and stylet immunolabeling results^a

Antibody ID	Peptide ID	AA positions in proteins	Peptide sequence	Specificity	Labeling	Accessibility
Anti-1-01	1-01	32–46	ILVQDSAPSADGSLK	Stylin-01	+	PAc
Anti-1-05	1-05	21–35	PAGQSPESRAVILVQ	Stylin-01	+	Em
Anti-1-06	1-06	38–52	APSADGSLKNNFQTD	Stylin-01	+	PAc
Anti-1-07	1-07	32–46	SQEQEVNFDGNFKNK	Stylin-02	+	PAc
Anti-1-08	1-08	48–62	NFQTDNGIKQEEVRY	Stylin-01	−	NA
Anti-1-09	1-09	59–73	EVRYLKAGPEGPVSV	Stylin-01	−	NA
Anti-1-10	1-10	92–106	YVADENGYQPYGAHL	Stylin-01	+	Em
Anti-1-11	1-11	121–135	RYLASLPSTPEPKYQ	Stylin-01/02	+	Ac
Anti-1-13b	1-13	108–122	TPPPIPAEIQESLRY	Stylin-01/02	+	Em
		106–120	LPTPPPIPAEIQESL

^aID, identifier; AA, amino acid; Ac, epitope exposed at the surface and directly accessible; PAc, epitope poorly accessible (labeling either weak or visible as dots without chitinase treatment); Em, epitope embedded, not accessible at the surface of the stylets (labeling visible only after chitinase digestion); NA, not applicable, because of the total lack of labeling regardless of the treatment. +, labeling observed at the tip of maxillary stylets; −, no labeling detected regardless of the tested conditions.

^bDue to poor immunogenicity prediction, two partially overlapping peptides were used for immunization.