Table 1.

Castanea mollissima DNA sample pools, with individuals (n) per sample site.

Pool	Location	Origin	na	Bases (quality clipped)	Estimated depth	Variant sitesb	Sites with alt >0.2c	Average Depthd
Y1	Yunnan- 26.013°N 101.0932°E	Forest	9	7,375,355,857	9.46	8,564,349	6,359,398	12.38
Y2	Yunnan- Fengqing County	Forest	10	11,619,019,171	14.89	11,431,073	9,371,773	17.56
S1	Shaanxi- Zhuque and Heihe Forests	Forest	13	7,344,176,568	9.42	13,492,467	8,279,766	9.46
S2	Shaanxi- Ningshan County	Forest	10	8,630,350,892	11.06	7,632,501	5,074,304	9.12
S3	Shaanxi- Liuba County	Forest	10	5,612,880,521	7.19	10,272,008	8,155,228	13.09
S4	Shaanxi-33.772° N 108.766° E	Orchard	10	9,890,960,153	12.68	17,086,140	12,681,410	13.96
GZ	Guizhou- 26.236° N 105.1676° E	Forest	10	8,594,920,262	11.02	10,046,443	7,949,407	14.10
HB	Hebei- 40.597° N 118.399° E	Orchard	10	6,175,704,628	7.92	7,796,678	5,479,063	11.03
BY	Beijing- Yanqing County	Orchard	10	5,240,552,948	6.72	6,844,594	4,301,595	9.03
ECC	Ohio, U.S.A.e	Orchard	12	3,498,422,441	4.49	4,939,429	2,079,207	7.10

^aNumber of individuals in pool.

^bSites with a variant called in a given pool with read depth >6.

^cSites with an alternate allele of frequency at least 0.2.

^dAverage observed depth at variant sites numbered in the 8th column.

^eGrown in Ohio, USA; derived from northern Chinese orchard cultivars.