Figure 2.

Cell-to-cell variation in isoform expression upon PMA stimulation. (A) Fluorescent images of three CD45 splicing isoforms visualized by SpliceRCA before and after PMA stimulation. (B) Histograms of the copy numbers of three mRNA isoforms in Jurkat T cells measured by SpliceRCA before and after stimulus. The arrow shows the expression shift; arrow to the right (left) means increased (decreased) expression upon PMA stimulation. (C) Quantification of average expression for three CD45 isoforms before and after stimulus. (D) Schematic diagram of the alternative splicing upon T-cell activation.