Mapping the spatial distribution of CD45 isoforms in the Jurkat
T cells. (A) Example images of the spatial distributions observed
for CD45 isoforms before and after PMA stimulation. The RCA amplicons
of CD45RO, CD45RA, and CD45RB are marked with green, blue, and red
circles, respectively, and the outlines of cell are marked by a gray
dotted line. Scale bars: 10 μm. (B) Single-cell profiles for
distance to cell nucleus (yellow dots) and the edge (black dots). N is the number of cell dots per plot. (C) Quantification
of average distances for three CD45 isoforms to the cell nucleus or
the edge before and after PMA stimulation.