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. 2018 Jun 14;4(6):760–767. doi: 10.1021/acscentsci.8b00257

Figure 2.

Figure 2

Biological activity of conjugates 6 and 7. (A) Dose response curves and IC50 (mean ± SE, n = 3) of conjugates 6 (black) and 7 (gray) in JIMT-1 cells obtained using an MTT-based assay. (B) Wnt/β-catenin luciferase reporter assay in Leading Light Wnt reporter cells. Columns show mean ± SE (n = 8). DMSO control = 100%. Cells were treated for 24 h. Firefly luciferase levels were normalized to Renilla luciferase levels. (C) Wnt/β-catenin luciferase reporter assay in JIMT-1 cells transfected with TOPFlash and Renilla plasmids. Cells were treated for 24 h. Columns show mean ± SE (n = 3). DMSO control = 100%. Firefly luciferase levels were normalized to Renilla luciferase levels. (D) Colony-forming efficiency (CFE) of JIMT-1 cells following treatment with conjugate 6 at IC50 (0.36 μM) for 72 h. Reported as percentage of colonies formed compared to DMSO control. Columns show mean ± SE (n = 3). (E) Inhibition of Wnt/β-catenin signaling in JIMT-1 cells. Cells were treated with the indicated concentrations for 72 h. Representative Western blots (n ≥ 3) used for densitometric scanning. SA, salinomycin (1). **P < 0.01; ***P < 0.001; ns, not significant.