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. 2018 Jun 14;4(6):760–767. doi: 10.1021/acscentsci.8b00257

Figure 3.

Figure 3

Cellular uptake and spectroscopic properties of conjugates. (A) Fluorescence microscopy experimental setup. (B) Fluorescence decay of conjugate 6 in various environments: ⟨τ⟩amp: cell = 2.7 ns; buffer = 1.2 ns; DMSO = 1.6 ns. (C) Representative data for real-time accumulation of conjugates 6 (black) and 7 (gray) (20 μM in HBSS with 2% DMSO) in individual JIMT-1 cells as monitored by fluorescence intensity. Fluorescence intensity flashes originating from aggregates of 7 during image capture (seen as gaps in the curve) were removed for clarity. (D) Fluorescence images of representative individual cells recorded during real-time accumulation experiments of conjugate 6 and 7. Scale bar = 20 μm.