Figure 4.

Imaging of fluorescent conjugate 6 in three breast cancer cell lines. Confocal and epifluorescence microscopy was carried out on JIMT-1, MCF-7, and HCC1937 cell lines. (A) Colocalization with ER-Tracker Red. Colocalization shown in orange/yellow. (B) Colocalization with Nile Red in LDs. The cells were incubated with Nile Red and images captured. The Nile Red was then bleached until no fluorescence was observed. Compound 6 was added to the medium of the cells, and images were captured ∼10 min after the imaging of Nile Red. (C, D) Absence of colocalization with Rhodamine 123 in mitochondria. The cells were incubated with Rhodamine 123, and images were captured. The Rhodamine 123 was then bleached until no fluorescence was observed. Compound 6 was added to the medium of the cells, and images were captured ∼10 min after imaging of Rhodamine 123. Panels C and D show maximum intensity projections and single optical planes, respectively. All images in panels A–D were obtained with a laser scanning confocal microscope. Scale bars = 10 μm. (E) DIC and fluorescence images of conjugate 6 in the three breast cancer cell lines were obtained with an epifluorescence microscope. Scale bar = 20 μm.