Figure 1.

Vav1‐dependent regulation of Akt1 in triple‐negative breast cancer‐derived cells. (A, B, C, D) Representative western blot analysis, performed with the indicated antibodies, of total lysates from MDA‐MB‐231 cells transfected with siRNA specific for Vav1 (Vav1 siRNA) or with a plasmid expressing human Vav1 (Over Vav1). Scramble siRNA (Ctrl siRNA) and an empty vector were used as control of the experiment. β‐Tubulin was blotted as an internal control of loaded proteins. Right histograms show the levels of the indicated proteins normalized to β‐tubulin. (E) MDA‐MB‐231 cells under the same experimental conditions were subjected to dynamic monitoring of proliferation using the impedance‐based xCELLigence Real‐Time Cell analysis (RTCA) system. Cell Index (CI) is reported, and error bars indicate ±SD. The correspondent slope analysis that describes the steepness, incline, gradient, and changing rate of the CI curves over time is shown on the right. All the data are the mean of three separate experiments ±SD. *P < 0.05.