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. 2018 May 21;12(7):1026–1046. doi: 10.1002/1878-0261.12308

Figure 1.

Figure 1

Increased proliferation of Sall2‐deficient cells. (A,B) Expression of Sall2 mRNA isoforms was evaluated in Sall2 wild‐type (Sall2 +/+) MEFs by RT/PCR (A) and qPCR (B) using Ppib as normalizer. P19 cells were used as positive control for SALL2 isoforms expression. (C) Sashimi plot (http://software.broadinstitute.org/software/igv/Sashimi) for alternatively spliced exon and flanking exon of Sall2 from deep RNA‐sequencing data of MEFs (SRX610348). Per‐base expression is plotted on y‐axis of Sashimi plot, genomic coordinates on x‐axis, and mRNA isoforms are represented on the bottom (exons in black, introns as lines). (D) Proliferation curves of Sall2 −/− and Sall2 +/+ MEFs. Equal number of MEFs (passage 3) was plated in triplicate and counted every day for 6 days. Data are expressed as mean ± SD from three independent experiments. *P ≤ 0.05, **P ≤ 0.01, Student's t‐test. (E) Cell cycle analysis of Sall2 −/− and Sall2 +/+ MEFs by flow cytometry. Asynchronous cells (passage 4) were collected, fixed, and stained with propidium iodide (PI) before cell cycle analysis. G0‐G1, S and G2‐M populations are indicated as percentages of the whole population. Figure is representative of two independent experiments of isogenic MEFs performed in triplicate.