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. 2018 May 21;12(7):1026–1046. doi: 10.1002/1878-0261.12308

Figure 5.

Figure 5

Immortalized Sall2‐deficient cells possess transforming ability. (A,B) Foci formation assay. iMEFs were grown in regular culture medium for 12–18 days prior to staining with crystal violet. (A) Microscopic visualization of three individual focus from Sall2 −/− iMEFs photographed at 4× magnification. The appearance of the Sall2 +/+ iMEFs culture (left) is shown for comparison.(B) Top, crystal violet staining of Sall2 +/+ and Sall2 −/− iMEFs. Bottom, quantification of number of foci per plate. Results are representative of three independent experiments performed in triplicate (**P ≤ 0.01, Student's t‐test). (C) Sall2 −/− MEFs showed increased anchorage‐independent growth. Sall2 +/+ and Sall2 −/− iMEFs were grown in soft agar for 3–4 weeks. Top, colonies were photographed at 4× magnification. Primary Sall2 +/+ MEFs were used as negative control. Bottom, quantification of number of colonies per plate. Results are representative of three independent experiments performed in triplicate (**P ≤ 0.01, Student's t‐test).