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. 2018 May 21;12(7):1104–1124. doi: 10.1002/1878-0261.12314

Figure 2.

Figure 2

MIST1 and PTF1a activate known target genes in PDAC cells. (A) Phase contrast images of Panc‐1 Tet‐MIST1 and Tet‐PTF1a cells ± Dox (scale bar = 50 μm). (B) RT‐qPCR analysis of Panc‐1 Tet‐MIST1 and Tet‐PTF1a cells. Data are normalized to the same cell lines without doxycycline treatment and displayed as fold induction. MIST1 vesicle trafficking target genes RAB3D and RAB26 and ER chaperones SEC61b, SEC62, SEC63, PERK, and DNAJc1 are induced upon MIST1 expression. (C) Expression of acinar transcription factors NR5a2, MIST1, GATA6, and PDX1 is induced in PDAC cells upon PTF1a induction. (D) Pancreas digestive enzymes PRSS1, PRSS2, CPA1, CPA2, CELA3b, and AMY2a transcripts accumulate upon PTF1a expression. (E) Trypsinogen (PRSS2) staining of Panc‐1 Tet‐One, Tet‐MIST1, and Tet‐PTF1a cells 72 h post‐doxycycline treatment (scale bar = 50 μm). (F) Percentage of Tet‐PTF1a cells expressing PRSS2 or carboxypeptidase (CPA1) upon doxycycline treatment. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.