Figure 7.

Localizations of Klp2p at different times in the cell cycle. (A) A diagram showing the organization of the pk-gfp tag that was integrated at the klp2⁺ locus to assure uniform levels of tagged protein expression driven by the klp2⁺ promoter. (B) Localization of Klp2p-GFP in logarithmically growing, interphase cells. Linear arrays of small spots are seen in the cytoplasm. (C) The Klp2p-GFP spots move along straight lines. The time after the beginning of the series is given in seconds for each exposure. The cell on the right lacks distributed dots and shows only one cluster of GFP staining. (D) Cells expressing Klp2p-GFP and vitally stained with Hoechst 33342. The positions of GFP (green) and DNA (purple) show that Klp2p-GFP localizes to the nucleus in cells with condensed or condensing chromatin. In early mitosis Klp2p-GFP localizes to several distinct spots within the nucleus. (E) In later mitosis, as indicated by the presence of two pools of DNA stain, a few spots are found located either in each nucleus or in a line between the nuclei. Bar, 4 μm.