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. 2018 Apr 24;7(2):47. doi: 10.3390/pathogens7020047

Figure 1.

Figure 1

Figure 1

Biofilm formation in dynamic conditions by P. aeruginosa PA14 exposed to C-type Natriuretic Peptide (CNP). (A) COMSTAT analyses of biofilms of P. aeruginosa PA14 control (blue bar) or exposed to several concentrations of CNP (orange bars). Data are the means of four measuring fields obtained from four independent experiments. ★★★, p < 0.001; NS: not significantly different. (B) 3D-shadow representations of the biofilm structures developed under dynamic conditions by P. aeruginosa PA14 control (left images) or exposed to CNP at 1 µM, 0.1 µM, 10 nM or 1 nM (right images). Biofilms were stained with the Syto 61 Red dye and observed by confocal laser scanning microscopy.