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. 2018 Jun 13;9(6):295. doi: 10.3390/genes9060295

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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C/EBPβ regulates the apoptosis and proliferation of ovarian GCs. PcDNA3.1-C/EBPβ decreased cell apoptosis (A) and increased cell proliferation (B) in granulosa cells as assessed by Annexin V-FITC/PI and Edu staining, respectively. SiRNA-C/EBPβ increased cell apoptosis (C,D) decreased cell proliferation in granulosa cells as assessed by Annexin V-FITC/PI and Edu staining, respectively. For Annexin V-FITC/P analysis, cells in the lower right quadrant were annexin-positive/PI-negative early apoptotic cells. The cells in the upper right quadrant were annexin-positive/PI-positive late apoptotic cells. The percentage of cells undergoing early and late apoptosis were presented in the relating barplot. ** indicates p < 0.01; * indicates p < 0.05. Data were represented as means ± SD.