Figure 7.

The GLUT4 retention compartment is only partially accessible to the TR in 3T3-L1 adipocytes. 3T3-L1 adipocytes expressing HA-GLUT4-GFP and either vpTR or the TR were incubated with Cy3 anti-HA, with or without HRP-Tf, and processed for the DAB reaction. The Cy3/GFP ratio (mean±SEM) was calculated and averaged over multiple cells. For both vpTR and the TR, the Cy3/GFP ratio with HRP-Tf is significantly different from the ratio with no HRP-Tf with p < 0.01 (heteroscedastic, two-tailed Student's t test).