Figure 5.

Dodecanoic acid (12 carbons) were mixed in a 1:1 mole ratio with its monoglyceride in pH 7.9 Bison Pool water, then were put through a wet-dry-wet cycle in the presence of 0.1 mM pyranine dye. Large vesicles are barely visible in the phase image (arrows, A) because the very thin bilayer membranes are not clearly resolved. However, the encapsulated pyranine was revealed within vesicles by fluorescence microscopy (B). Some vesicles have excluded the dye for reasons explained in the text.