Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) mRNA was extracted from MLO-Y4 cells treated with CHIR99021 at 100 nM for 48 H. The expression of Sclerostin gene (Sost) was evaluated by RT-PCR. (B) A replicate of same samples were extracted for immunoblotting procedure. 40 μg of protein extracts were separated in SDS-PAGE and Sclerostin protein was revealed.
