Box plot indicating lengths of scaffolds included and excluded from Reference-Assisted Chromosome Assembly’s (RACA’s) assembled fragments. RACA was provided with the largest 500 scaffolds, which ranged from 48 to 55,683 Kbp. The scaffolds included in the assembly spanned approximately the full range of sizes (50 to 55,683 Kbp) whereas the excluded scaffolds tended to be smaller (48 to 656 Kbp). The outlier points shaded in blue represent scaffolds 292 and 310, which were experimentally demonstrated to contain red fox Y-chromosome sequence [14], and therefore would not be expected to be included in RACA’s assembled fragments.