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LC-MS chromatographic separation profile of the putative peptides (top) (A) OP3-4 (m/z 1450.9) and (B) DOTA-OP3-4, (m/z 2081.4) with their respective ITMS ion mass spectra (bottom) eluted as peaks at 11.2–11.5 and at 25.3 min. The purity of the putative peptides, estimated by integration of the peak areas, was >95% (OP3-4) and 90% (DOTA-OP3-4).
