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T1 weighted MRI scan of DOTA-Gd-OP3-4 showing a concentration-dependent signal intensity. The peptides, pre-dissolved in DMSO, were diluted to the desired concentration in PBS buffer (1 mL) and the MRI signal acquired at the same time using the parameters: SL5, TE 8.7, and TR550. DOTA-Gd (MW 557.64 g/mol) and DOTA-Gd-OP3-4 (MW 2141.4 g/mol) were prepared at a concentration of 1 µg/ml, corresponding to 1.79 µM and 0.47 µM Gd, respectively.
