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. 2018 Jun 2;8(6):399. doi: 10.3390/nano8060399

Figure 6.

Figure 6

OP3-4, DOTA-OP3-4, and DOTA-Gd-OP3-4 have no significant effect on the viability of SAOS-2 cells. (A) The cells were cultured with different concentrations of OP3-4 peptide for 24 h and the percentage of dead and live cells determined by assaying for LDH in supernatant and MTS assay, respectively. Absorbance was measured at 490 nm. (B) The effect of derivatised OP3-4 peptides (at 100 µM) on cell viability was determined by fluorescence microscopy after H/PI live and dead cell staining (B). Data is mean ± SE, n = 6.