Figure 1.

Microfluidic hydrodynamic focusing (MHF) methods used to prepare liposomes or vesicles. (a) Schematic of an MHF device used to prepare liposomes; (b,c) Biomimetic nanovesicles were prepared by the MHF method. (b) Schematic diagram of device, (c) in vitro and in vivo biological properties of liposome-like nanovesicles (NA-Leuko), (i) in vivo inflammatory targeting of NA-Leuko in localized ear inflammation mold, (ii) quantification of NA-Leuko, into the inflamed ear compared to healthy ears at different time after injecting, (iii) consequence of flow cytometry (6 and 24 h incubation) in vitro uptake studies of control liposomes and murine NA-Leuko following incubation with J774 macrophages (reprinted with permission from [36]).