Skip to main content
. 2018 Jun 25;9:614. doi: 10.3389/fphar.2018.00614

FIGURE 4.

FIGURE 4

BHDPC promoted microglia polarization to the M2 phenotype in LPS-stimulated BV-2 microglial cells. Cells were treated with BHDPC for 1 h, followed by LPS stimulation. The cytokine level was determined by Elisa. The mRNA and protein expression were determined by qPCR and Western blot assay, respectively. (A–C) BHDPC increased M2 marker IL-10, CD206, and Arg-1 expression in BV-2 cells. (D–F) BHDPC prevented the LPS-induced down-regulation of YM1/2, CD206, and Arg-1 in mRNA level. The mRNA level was normalized to GAPDH and expressed as fold- increase. Data are presented as means ± SEM of three independent experiments in triplicate. Control group was untreated cells. #P < 0.05 and ##P < 0.01, versus control group; ∗P < 0.05 and ∗∗P < 0.01, versus LPS-treated group.