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. 2018 Jun 25;9:614. doi: 10.3389/fphar.2018.00614

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Copyright © 2018 Li, Chen, Zhou, Feng, Hoi, Ma, Zhao, Zheng and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BHDPC provided neuroprotective effects against the HT22 cells damages induced by LPS-activated BV-2 microglia. BV-2 cells were co-cultured on Transwell inserts with HT22 cells. Microglial were pretreated with BHDPC for 1 h, and then stimulated with LPS. (A) The cell viability was measured via MTT assay. (B) Cellular morphology of HT22 cells. (C,D) The cellular ROS level and caspase 3 activity were measured via the fluorogenic probe and Caspase-3 Assay Kit, respectively. (E) Cell DNA fragmentation was measured via ELISA assay. (F,G) The mitochondrial membrane potential (MMP) was detected using JC-1 dye. Data are presented as means ± SEM of three independent experiments in triplicate. Control group was untreated cells. ^#P < 0.05 and ^##P < 0.01, versus control group; ^∗P < 0.05 and ^∗∗P < 0.01, versus LPS-treated group.