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. 2018 Mar 27;6(2):16. doi: 10.3390/proteomes6020016

Figure 3.

Figure 3

Western blot analysis of Cytosolic and mitochondrial fractions of HCC1937 and MCF7 cancer cells. Left panel: (a) HSP60 resulted upregulated in the whole extract of hereditary breast cancer cells (HCC1937) compared to sporadic breast cancer cells (MCF7); Western blot signals were normalized using α-tubulin as housekeeping proteins. (b) HSP60 was down-expressed in the cytosolic fraction of hereditary cancer cells (HCC1937) compared to sporadic breast cancer cells (MCF7); Western blot signals were normalized using α-tubulin as housekeeping proteins. (c) HSP60 was upregulated in the mitochondrial fraction of hereditary breast cancer cells (HCC1937) compared to sporadic breast cancer cells (MCF7); Western blot signals were normalized using cyt c as housekeeping proteins. Right panel: Densitometry analysis for each analyzed proteins. Analysis was performed using three independent experiments. Data are mean ± SEM (N = 3) p < 0.05. For each western blot 50 µg of proteins were resolved by SDS-PAGE using Any kD™ Mini-PROTEAN precast gels and electrotransferred to a nitrocellulose membrane with a Trans-blot turbo system (Biorad). Images were acquired using the Alliance 2.7 system (UVITEC, Eppendorf, Milan, Italy) and analyzed by excel spreadsheet.