Table 1.
Comparison of oxygen consumption rate/oxygen uptake of the whole cell catalyst (in grey) with values for pure dioxygenases found in literature (in blue) [36,37].
| Biocatalyst | Substrate | Amount of Oxygenase Component of NDO | Measurement Setup | Oxygen Uptake (mM·min−1) |
|---|---|---|---|---|
| NDO from Sphingomonas CHY-1 [37] | Naphthalene (0.1 mM) |
PhnI (0.13 µM) |
Clark-type oxygen electrode | 14.95 |
| NDO from Pseudomonas sp. Strain NCIB 9816-4 [36] | Styrene (0.1 mM) |
ISPNAP
(25 µg) |
0.0700 | |
| NDO (pDTG141) wild-type in E. coli JM109/DE3) | Styrene (1 mM) |
- | Luminescent oxygen sensors | 0.3202 |
| NDO variant V260A in E. coli JM109/DE3) | Styrene (2 mM) |
0.6553 | ||
| NDO variant H295A in E. coli JM109/DE3) | Styrene (2 mM) |
0.4560 |
NDO: Naphthalene dioxygenase.