Figure 1.

Directed differentiation of MSC expanded in synthetic media. MSC were expanded in each of the synthetic media as described in Table 1. At the end of 4 weeks of culture, the cells were harvested, counted, and re-plated in differentiation media for a subsequent 3 weeks. At that time, the cells were analyzed either for osteogenic (top panel) or adipogenic differentiation (bottom panel) by histology. Magnification for all photographs is 10×.