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. 2018 Jun 14;8(6):433. doi: 10.3390/nano8060433

Figure 4.

Figure 4

The gene silencing efficacy of PAMAM-GATG-HA4 nanocomplexes analyzed by flow-cytometry and quantitative real-time polymerase chain reaction (qRT-PCR) in MDA-MB-231-GFP cells. The gene silencing efficacy without adding hyaluronan tetrasaccharide was analyzed (a) by flow-cytometry and (b) with adding hyaluronan tetrasaccharide analyzed by flow-cytometry. (c) The gene silencing efficacy without adding hyaluronan tetrasaccharide analyzed by qRT-PCR and (d) with adding hyaluronan tetrasaccharide analyzed by qRT-PCR. Statistical analysis was performed with one-way ANOVA and Bonferroni post-hoc testing with * p < 0.05 and *** p < 0.01. The “negative” represents the blank control group using MDA-MB-231 cells without GFP; the “control” means the MDA-MB-231-GFP cells were not treated with siGFP or nanocomplexes; and the “free” and “lipo” mean the MDA-MB-231-GFP cells were treated with free siGFP or lipofectamine 2000 containing siGFP respectively.